Part:BBa_K188774
no.1
To inhibit transcription of the LacI promoter (pLacl, BBa_R0010), we have to encode LacI protein (BBa_C0012). LacI protein can bind to pLacI and repress the expression, but lactose, which repress lacI binding, act as a switch opening the expression. Therefore, the inter-regulation could be a timer device, so LacI is a key component of first device. The second gene is the LuxR, which is a membrane receptor, takes external AHL compound to pass down the signals. We use the LuxR as our bacteria-sensor to sense other bacteria in the environment. For selecting other parts, we choose one kind of constitutive promoter family(BBa_J23106) as the promoter, and the expression amount of this promoter is moderate comparing others in the family. Furthermore, we use the commom ribosome-binding site(BBa_B0034) and the terminator(BBa_B0014), which is a special double terminator, to ensure the transcription would exactly stop.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2029
- 1000COMPATIBLE WITH RFC[1000]
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